基质细胞衍生因子-1α在增生性糖尿病视网膜病变继发新生血管性青光眼中的作用

摘要

目的 观察基质细胞衍生因子-1α(SDF-1α)在增生性糖尿病视网膜病变(PDR)继发新生血管性青光眼(NVG)中的作用,并探讨其作用机制.方法 采集PDR患者25例31只眼的玻璃体标本.其中,继发NVG者13只眼作为实验组,无NVG者18只眼作为对照组.配制含10、100、1000 ng/ml SDF-1α和10 ng/m1血管内皮生长因子(VEGF)培养液,并以此分组;测量各浓度组与体外对照组人脐静脉内皮细胞(HUVEC)管腔样结构及毛细血管样结构全长.配制含10、100、1000 ng/ml SDF-1α和100 ng/ml血管内皮生长因子(VEGF)培养液,并以此分组;采用5'-溴2'-脱氧尿嘧啶(BrdU)掺入法行细胞增生检测,分析各浓度组与体外对照组吸光度[A,旧称光密度(OD)]值.采用酶联免疫吸附试验(ELISA)检测玻璃体标本实验组和玻璃体标本对照组患者玻璃体标本中VEGF和SDF-1α含量.结果 体外血管生成检测显示,10、100、1000 ng/ml SDF-1α和10 ng/ml VEGF组HUVEC管腔样和毛细血管样结构长度均较体外对照组长,差异均有统计学意义(P＜0.05).细胞增生检测显示,10、100、1000 ng/ml SDF-1α和100 ng/ml VEGF 组A值均较体外对照组增高,差异有统计学意义(P＜0.05).ELISA法检测显示,玻璃体标本实验组患者玻璃体标本中SDF-1α和VEGF含量均明显高于玻璃体标本对照组,差异有统计学意义(P＜0.01).结论 SDF-1α参与了PDR继发NVG的形成过程,可能与SDF-1α促进血管内皮细胞增生而促进新生血管形成有关.%Objective To observe the effects of stromal cell-derived factor 1α (SDF-1α) in secondary neovascular glaucoma (NVG) of proliferative diabetic retinopathy (PDR). Methods The vitreous specimens from 25 PDR patients (31 eyes) were collected with 13 NVG eyes and non-NVG 18 eyes. The concentrations of SDF-1α and vascular endothelial growth factor (VEGF) in those specimens were measured by enzyme-linked immunosorbent assay (ELISA). Human umbilical vein endothelial cells (HUVEC) were treated by different concentrations of SDF-1αand vascular endothelial growth factor (VEGF) in vitro, and the formation of tube cavity-like structure, length of capillary-like structures and 5'-bromo-2'-deoxyuridine (BrdU) labeling of treated HUVEC were measured. Results The length of HUVEC tube-like and capillarylike structure formation in 10, 100, 1000 ng/ml SDF-1α and 10 ng/ml VEGF groups were longer than that in the control group, the differences were statistically significant (P＜0. 01). The A value of BrdU labeling of 10, 100, 1000 ng/ml SDF-1α and 10 ng/ml VEGF groups were increased than that in the control group,the differences were statistically significant (P＜0.01). The vitreous levels of SDF-1α and VEGF of NVG specimens were higher than those in the non-NVG group, the differences were statistically significant (P＜0.01). Conclusions SDF-1α may promote the migration and proliferation of vascular endothelium cells, and participate in the neovascularization process in NVG patients with PDR.