链状酰胺类化合物B07的遗传毒性试验研究

摘要

目的:探讨链状酰胺类化合物B07是否存在遗传毒性.方法:通过Ames试验、中国仓鼠肺成纤维细胞(CHL)染色体畸变试验和大鼠骨髓嗜多染红细胞微核试验,研究B07的遗传毒性.结果:Ames试验中,B07在每皿5 000 μg的浓度时,诱发TA102(±S9)菌株产生的回变菌落数高于空白对照组(P＜0.01);在每皿0.5 ～ 500 μg内,未见TA102回复突变率增高;在每皿0.5～5 000 μg内,未见TA97,TA98,TA100和TA1535回复突变率增高.染色体畸变试验中,B07浓度在75 ～ 600μg· mL-1,未见诱发CHL细胞染色体畸变率增高.微核试验中,B07浓度在1.6 ～6.4 mg·kg-1无致大鼠骨髓嗜多染红细胞微核形成作用.结论:高浓度B07可能有致突变作用.%Objection:To evaluate the genotoxicity of chainamide compounds B07.Methods:The genotoxicity of B07 was studied by Ames test,chromosomal aberration test in Chinese hamster lung (CHL) cell and micronucleus test in rat bone marrow.Results:In Ames test,at concentration of 5 000 μg· plate-1,the reversal colonies in TA102 were increased (P ＜ 0.01) compared with negative control.At concentration of 0.5 ～ 500 μg·plate-1,the reversal colonies in TA102 were not increased.At concentration of 0.5 ～ 5 000 μg·plate-1,the reversal colonies in TA97,TA98,TA100 and TA1535 were not increased.In chromosomal aberration test,B07 did not induce a significant increase in the rate of CHL cell chromosomal aberrations at concentration of 75 ～ 600 μg· mL-1 Micronucleus test indicated that B07 had no mutation effect on the rate of rat micronucleated polychromatic erythrocytes at dose of 1.6 ～ 6.4 mg· kg-1.Conclusion:B07 in high dose may cause gene mutation.