Objective To study the inhibition mechanism of triethyltin chloride (TETC) on proliferation of C6 glioma cells in vitro. Methods Inhibitory rate of TETC on the proliferation of C6 glioma cells , apoptotic rate of C6 glioma cells and expression of Bcl-2 and Bax protein were tested by MTT assay, fluorescent TUNEL assay and Western blotting analysis at 48 h after treated by different concentrations of TETC on C6 glioma cells, respectively. Results The proliferation of C6 glioma cells was inhibited by TETC in a dose-dependent manner. TETC induced apoptosis of C6 glioma cells and the apoptotic rate was increased in a duse-dependent manner. The expressions of Bcl-2 and Bax protein were down-regulated and up-regulated, respectively. Conclusions TETC could inhibit the proliferation of C6 glioma cells in vitro. TETC could induce the apoptosis of C6 glioma cells, decrease the expression of Bcl-2 protein, increase the expression of Bax protein, lead to apoptosis of C6 glioma cells, and these maybe the mechanism of anti-proliferation of TETC.%目的 探讨氯化三乙基锡(TETC)对C6胶质瘤细胞体外增殖抑制作用的机制.方法 采用MTT法、荧光原位末端标记方法和Western印迹方法,分别检测不用浓度TETC作用C6胶质瘤细胞48 h后细胞增殖抑制率、细胞凋亡率和Bcl-2和Bax蛋白的表达水平.结果 TETC对C6胶质瘤细胞具有抑制增殖作用,增殖抑制率呈剂量依赖性；TETC可诱导C6胶质瘤细胞凋亡,细胞凋亡率呈剂量依赖性上升；TETC可下调Bcl-2和上调Bax蛋白表达水平.结论 TETC可抑制C6胶质瘤细胞体外增殖并诱导其凋亡,机制可能与其下调Bcl-2和上调Bax蛋白表达有关.
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