Objective To investigate the effects of the adenovirus expression vector of small interfering RNA ( siR-NA) targeting vascular endothelia growth factor receptor 3 (VEGFR-3) gene on apoptosis and invasion of human colon cancer LoVo cells. Methods VEGFR-3 siRNA adenovirus was transfected into the LoVo cells, and the expressions of VEGFR-3 were measured by Western blotting. The apoptosis was detected by Hoechst 33342 staining and FCM ( flow cytometry) . The invasion ability was assayed by Transwell method. Results Compared with the blank control group and the negative control group, the expression level of VEGFR-3 protein in LoVo cells of experimental group was re-duced after transfection with pAd-VEGFR3-siRNA ( P <0 . 05 ) . The apoptosis rate was increased significantly ( P <0. 05). The proliferation of LoVo cells was inhibited, the invasion ability decreased sinificantly (P<0. 05). Conclu-sion VEGFR-3 siRNA can down-regulate the expression levels of VEGFR-3 protein in LoVo cells, inhibit the invasion abilities of LoVo cells, and induce the apoptosis of LoVo cells effectively. VEGFR-3 can be used as a potential aim of colon cancer targeted therapy.%目的：探讨靶向血管内皮细胞生长因子受体3(vascular endothelial growth factor receptor-3, VEGFR-3)基因的小干扰RNA ( small interfering RNA,siRNA)腺病毒载体对人结肠癌LoVo细胞系凋亡及侵袭的影响。方法将靶向VEGFR-3 siRNA腺病毒转染结肠癌LoVo细胞,以Western blotting检测VEGFR-3蛋白的表达,Hoechst 33342染色法和流式细胞仪检测LoVo细胞的凋亡情况,用Transwell小室测定LoVo细胞的侵袭力。结果实验组与空白对照组和阴性对照组比较,实验组中转染靶向VEGFR-3 siRNA腺病毒后结肠癌LoVo细胞中VEGFR-3蛋白的表达被下调(P<0.05)。 Hoechst 33342染色法和流式细胞仪检测LoVo细胞凋亡率明显升高(P<0.05),Transwell小室测定LoVo细胞侵袭能力下降(P<0.05)。结论靶向VEGFR-3 siRNA腺病毒能下调结肠癌Lo-Vo细胞中VEGFR-3蛋白的表达,从而诱导结肠癌LoVo细胞的凋亡,抑制其侵袭能力,因此,VEGFR-3可以作为结肠癌靶向治疗的一个潜在靶点。
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