一株致婴儿死亡的腺病毒Ad7d2的基因特点

摘要

目的 对一株致婴儿死亡的腺病毒Ad7d2的基因特点进行分析.方法 对2006年北京儿童医院1名住院儿童(治疗过程中死亡)的鼻咽分泌物进行病毒分离,对病毒核酸进行六邻体和纤维部分BJ060316-1,经PCR扩增以及序列测定和分析确定为腺病毒Acr7d2.结果 对其六邻体和纤维基因进一步分析发现BJ060316-1与Ad7d2同源性最高,六邻体基因950 bp片段与以色列1993年分离株Ad7d2原型株AF321311核苷酸同源性为99.5%,对推断的氨基酸序列比对发现,BJ060316.1在253位缺失了谷氨酰氨,而在495位的丝氨酸被精氨酸代替.扩增的975 bp纤维基因片段与日本2005年Ad7分离株AB243118核苷酸同源性为99.7%.结论 腺病毒Ad7d2分离株BJ060316-1未发现毒力位点突变.%Objective To analyze.the genetic characteristics of a strain of adenovirus,Ad7d2,isolated from an infant died of severe pneumonia.Methods Virus isolation was performed by using the nasopharyngeal secretion from an 11-month-old infant with serious pneumonia.The viral DNA Was amplified by PCR and the products were sequenced.Results One strain of virus was isolated and Was narded as BJ060316-1.Sequence analysis of the hexon and fiber gene of the PCR products showed that the strain was Ad7d2.which shared 99.5% homology for 950bp hexon fragment with AF321311 Ad7d2 isolated from Israel in 1993.Blast with deduced amino acid sequence showed that BJ0603 16-1 lost glutamine at site 253.and at the site 495 arginine replaced serine.For fiber gene, BJ060316-1 showed 99.7%homology with AB2431 18 Ad7 isolated in Japan in 2005 for 975 bp fragment.Conclusion Adenovirus Ad7d2 strain BJ0603 16-1 isolated from a an infant with fatal pneumonia showed no virulence mutation.