人牙周膜成纤维细胞与牙龈成纤维细胞差异表达基因的批量克隆及其特征分析

摘要

AIM: To clone and analyze the functional characteristics of genes expressed differenty in cultured primary human PDLF in comparison with GF. METHODS: Subtractive cDNA library of PDLF was constructed with a modified gene cloning technique which is based on PCR and subtractive hybridization. Genes known to GeneBank were analyzed concerning their functional characteristics. RESULTS: 14 genes were cloned and the 10 known genes are responsible for intracellular process of cell differentiation and matrix synthesis and secretion. CONCLUSION: This study suggests the possible potential of PDLF to differentiate and comparatively more active intracellular protein synthesis and secretion.%目的：克隆人牙周膜成纤维细胞(periodontal ligament fibroblast, PDLF)rn与牙龈成纤维细胞(gin-gival fibroblast, GF)差异表达基因并初步分析其中已知基因的功能特征。方法：采用基于PCR和消减杂交的基因克隆技术构建人PDLF与GF差异表达基因的扣除文库，克隆人PDLF与GF差异表达基因，对已知基因的功能特征进行分析。结果：成功克隆到14个人PDLF与GF细胞差异表达基因，其中10个为已知基因。已知基因的功能多与细胞分化和细胞外基质的合成、分泌有关。结论：牙周膜成纤维细胞相对于牙龈成纤维细胞可能具有一定的分化潜能和相对旺盛的蛋白合成与分泌活性。