人牙周膜成纤维细胞与牙龈成纤维细胞差异表达一条新基因的克隆

摘要

AIM: To clone a new gene that is differentially expressed in human PDLF in comparison with GF.METHODS: Subtractive cDNA DNA library of PDLF was constructed with a recently developed gene cloning technique that is based on PCR and subtractive hybridization and then screened by restriction analysis and reverse southern dolt blot. Desired genes were sequenced and then analyzed by computer. RESULTS: We cloned a 818bp new gene from a PDLF cDNA subtractive library. CONCLUSION: We cloned a new gene from the subtuctive htnan PDLF cDNA library.%目的：克隆人牙周膜成纤维细胞( periodontal ligament fibroblast, PDLF)rn与牙龈成纤维细胞(gin-gival fibroblast, GF)差异表达的新基因。方法：采用基于PCR和消减杂交的基因克隆技术构建体外培养的人PDLF与GF差异表达基因的扣除文库，采用酶切和反向杂交的方法筛选目的克隆，并进行测序和计算机分析。结果：从构建的人PDLF与GF细胞差异表达基因的扣除文库中筛选到818bp新基因。结论：采用基于PCR和消减杂交的基因克隆技术获得一条人PDLF与GF间差异表达新基因。