组蛋白乙酰化修饰调控RAR-β2表达与宫颈病变的相关性

摘要

目的：探讨组蛋白乙酰化修饰与RAR-β2表达及宫颈病变发生发展的关系。方法：收集正常宫颈、宫颈上皮内瘤变（cervical intraepithelial neoplasia，CIN）Ⅱ～Ⅲ和宫颈鳞状细胞癌（squamous cell carcinoma，SCC）组织，分别采用免疫组织化学和Western Blot方法检测AcH3、RAR-β2和Involucrin的表达；分析组蛋白乙酰化水平与RAR-β2表达及宫颈病变程度的关系。结果：随着宫颈病变的进展，AcH3、RAR-β2和Involucrin的表达逐渐降低，甚至缺失，组间差异有统计学意义（P<0.05）；组蛋白乙酰化水平与RAR-β2表达呈正相关（r=0.797，P<0.05），AcH3和RAR-β2的表达均与宫颈病变程度呈负相关[r=-0.547（AcH3），r=-0.585（RAR-β2）， P<0.05]。结论：组蛋白乙酰化修饰与RAR-β2表达调控有关，并可能参与宫颈癌的发生。%Objective:To investigate the relationship between cervical lesion development and histone acetylation that regulates RAR-β2 expression. Methods:Immunohistochemistry and Western blot analysis were performed to detect AcH3, RAR-β2, and involu-crin expression in normal cervical tissues as well as in tissues with cervical intraepithelial neoplasia (CIN)Ⅱ-Ⅲand squamous cell cer-vical carcinoma. The relationship among histone acetylation level, RAR-β2 expression, and cervical lesion severity were analyzed. Re-sults:AcH3, RAR-β2, and involucrin expression were reduced or absent with the progression of cervical lesions;significant differences were noted between the groups (P<0.05). Histone acetylation level and RAR-β2 expression were positively correlated (r=0.797, P<0.05). AcH3 and RAR-β2 expression, which were both associated with the cervical lesions, were negatively correlated [r=-0.547(AcH3), r=-0.585(RAR-β2), P<0.05]. Conclusion:Histone acetylation modification is associated with the regulation of RAR-β2 expression. This pro-cess is also likely to participate in the carcinogenesis of cervical carcinoma.