MODULATION OF GENE EXPRESSION BY MODULATING HISTONE ACETYLATION

摘要

The present invention provides a composition, which contains a first agent that affects histone acetylation and a second agent that induces gene expression through a phosphorylation dependent response element binding factor. The invention also provides a method of modulating the phosphorylation state of a response element binding factor by contacting a cell containing the binding factor with an agent that affects histone acetylation, thereby modulating the phosphorylation state of the response element binding factor. The present invention further provides a method of ameliorating a pathologic condition by potentiating expression of a gene regulated by a phosphorylation dependent response element binding factor in a subject, by administering to the subject a pharmaceutical composition containing a first agent that affects histone acetylation and a second agent that induces expression of the gene through the phosphorylation dependent response element binding factor. The invention also provides a method of altering expression of a nucleosomal organized nucleic acid molecule by modulating attenuation of expression of the nucleic acid molecule, which contains an expressible nucleotide sequence operably linked to an inducible response element, which is bound in a phosphorylation dependent manner by a response element binding factor. Also provided is a method of identifying an agent that modulates attenuation of expression of a nucleic acid molecule.