原位衍生化技术在液相色谱和液相色谱-质谱联用分析中的应用

摘要

衍生化是将待分析物转化为更适合的物质形式以便于分析的有效手段.原位衍生化技术作为一种常用的柱前衍生化方法,可以在样品基质中同时完成分析物的萃取和衍生化,具有高效、灵敏和选择性好的优点.原位衍生化结合其他前处理技术广泛用于胺类、醛酮类、醇类、酚类、羧酸和巯基化合物的分析中,在生物、药物、食品、环境、化妆品分析等领域有广泛的应用.该文概述了原位衍生化的反应类型和代表性衍生试剂,综述了原位衍生化技术在液相色谱和液相色谱-质谱联用分析中的应用,并展望其发展趋势.%Derivatization is an effective method to analyze substances,in which the analyte is converted into a more suitable form for analysis.As one of the most used pre-column derivat-ization methods,in situ derivatization can simultaneously extract and derivatize the analyte in a sample matrix,resulting in good efficiency,sensitivity,and selectivity of the analytical method.Recently,in situ derivatization combined with sample pretreatment techniques has been widely used in biological,drug,food,environmental,and cosmetic samples for the analysis of trace amines,aldehydes and ketones,alcohols,phenols,carboxylic acids,and thiols.This review summarizes the reaction types and representative derivatization reagents of in situ derivatiza-tion.The applications of in situ derivatization in liquid chromatography( LC)and liquid chroma-tography-mass spectrometry( LC-MS)analysis are examined.Furthermore,the long-term pros-pects and potential applications of in situ derivatization are discussed.