桦木酸对环磷酰胺致小鼠免疫器官氧化损伤的保护作用

摘要

The objective of this research was to evaluate the effects of betulinic acid (BA) on immune organs of oxidative damaged mice induced by cyclophosphamide (Cy).Fifty male healthy Kunming mice were randomly divided into 5 groups,which were control group,Cy group,and 0.05,0.50 and 5.00 mg/kg BW BA groups.Control and Cy groups were orally administered with 1％ starch solution,and the other groups were orally administered with different doses of BA for 14 days.Except control group,mice in the other groups were intraperitoneal injected Cy at the dosage of 50 mg/kg BW to set up oxidative damage model.Serum,spleen and thymus of mice were collected.Activities of alanine aminotransferase (ALT) and aspartate transaminase (AST),contents of total protein (TP) and albumin (ALB) in serum,as well as activities of superoxide dismutase (SOD),glutathione peroxidase (GSH-Px) and catalast (CAT),and contents of glutathione (GSH) and malondialdehyde (MDA) in spleen and thymus were determined.The results showed as follows:1) about body weight,control group and Cy group had no significant difference (P＞0.05),and BA groups had no significant difference with Cy group (P＞0.05).2) Compared with control group,Cy significantly increased serum AST and ALT activities (P＜0.01),and 0.05 mg/kg BW BA significantly relieved the effects of Cy (P＜ 0.01).3)Compared with control group,Cy significantly decreased thymus index (P＜0.05),and 0.05 mg/kg BW BA significantly relieved the effects of Cy (P＜0.05).4)Compared with control group,Cy significantly decreased SOD and CAT activities in thymus,and GSH content in spleen (P＜0.05 or P＜0.01),significantly increased spleen MDA content (P＜0.01);compared with Cy group,0.05 mg/kg BW BA group had significantly higher GSH-Px and CAT activities in spleen and thymus (P＜0.05 or P＜0.01),and significantly lower MDA content in spleen (P＜0.01),but had significantly lower SOD activity in thymus (P＜0.01);compared with Cy group,5.00 mg/kg BW BA group had significantly higher GSH-Px and CAT activities in spleen and thymus(P＜0.05 or P＜0.01),and significantly lower MDA content in spleen (P＜0.01).The results suggest that BA can effectively improve oxidative stress of immune organs and shows preventive protection of oxidative damage in mice induced by Cy.%为了研究桦木酸(BA)对环磷酰胺(Cy)诱导小鼠免疫器官氧化损伤的影响.将50只健康雄性昆明小鼠随机分为5组,即对照组、Cy组及0.05、0.50、5.00 mg/kg BW BA组.对照组和Cy组灌服1％的可溶性淀粉溶液,其余各组按不同剂量的BA灌服,连续14 d后,除对照组注射生理盐水外,其余4组均腹腔注射Cy(50 mg/kg BW)诱导氧化应激模型.收集血清、脾脏和胸腺,检测血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)活性及白蛋白(ALB)、总蛋白(TP)含量,检测脾脏和胸腺超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)活性及还原型谷胱甘肽(GSH)、丙二醛(MDA)含量.结果显示:1)对于体重,对照组与Cy组无显著差异(P＞0.05),各剂量BA组与Cy组无显著差异(P＞0.05).2)与对照组相比,Cy极显著提高了血清AST、ALT活性(P＜0.01),而5.00 mg/kg BW BA极显著缓解了这一现象(P＜0.01).3)与对照组相比,Cy引起了脾脏指数和胸腺指数的显著降低(P＜0.05),而0.05 mg/kg BW BA显著缓解了胸腺指数的降低(P＜0.05).4)与对照组相比,Cy组胸腺SOD活性、胸腺CAT活性、脾脏GSH含量显著或极显著下降(P＜0.05或P＜0.01),脾脏MDA含量极显著升高(P＜0.01);与Cy组相比,0.50 mg/kg BW BA组显著或极显著提高了脾脏和胸腺GSH-Px和CAT活性及脾脏GSH含量(P＜0.05或P＜0.01),极显著降低脾脏MDA含量(P＜0.01),但极显著降低了胸腺SOD活性(P＜0.01);与Cy组相比,5.00 mg/kg BW BA组显著或极显著提高了脾脏和胸腺GSH-Px和CAT活性(P＜0.05或P＜0.01),极显著降低脾脏MDA含量(P＜0.01).由此可见,BA能够改善Cy引起的小鼠免疫器官的氧化应激,对Cy诱导的氧化损伤有预防性的保护作用.