Protective Effects of Quercetin derivatives against Hydrogen Peroxide-induced Oxidative Damage in PC-12 Cells

摘要

In order to search for new structural modification strategies on quercetin, we have designed and synthesized a series of quercetin derivatives. Several novel quercetin derivatives displayed potent protect effect on H_2O_2-induced injury in PC 12 cells. In the recent study, we investigated the effect of 3,3',4',5,7-pentahydroxyflavone 2-(3,4-dihydroxyphenyl)-3,5,7- trihydroxy-4H-l- benzopyran -4-one (HPS5) on the apoptosis of PC12 cells in vitro. We used neuronal PC12 cell line to study the regulation role of HPS5 in H_2O_2 induced cell apoptosis. MTT assay was used for detection the proliferation of PC 12 cells upon H_2O_2 treatment. PC 12 cell nuclear morphological change was detected with Hoechst 33258 staining. The antioxidative capacity was judged by SOD determinations. The protein levels of Bax and Bcl-2 were deteanined by Western blotting. Our results demonstrated that Quercetin derivatives HPS5 can prevent and protect against H_2O_2-induced injury in PC 12 cells. Together, our studies provided new insights activities of quercetin, and may contribute to pharmacological study of quercetin in future research.