痰热清注射液联用左氧氟沙星（ig/iv）后小鼠肺组织中左氧氟沙星的分布比较

摘要

目的：比较痰热清注射液联用左氧氟沙星（ig/iv）后小鼠肺组织中左氧氟沙星的分布。方法：选用144只小鼠，随机分为对照组和实验组，每组72只。所有实验小鼠均行iv痰热清注射液（0.8 ml/kg），1 h后，对照组小鼠iv左氧氟沙星注射液，实验组小鼠ig左氧氟沙星片溶液，剂量均为80 mg/kg。分别于给药后0.05、0.083、0.17、0.5、1.0、2.0、4.0、8.0、12.0 h和0.083、0.17、0.33、0.5、1.0、2.0、4.0、8.0、12.0 h采集肺组织，每个时间点取8只小鼠。以环丙沙星为内标，采用高效液相色谱法测定肺组织中左氧氟沙星的含量，3p97软件计算药动学参数。结果：实验组和对照组小鼠肺组织中左氧氟沙星的药-时曲线均符合二室模型，t1/2β分别为（4.17±0.84）、（4.10±0.55）h，CL分别为（0.66±0.049）、（0.71±0.21）L/h，AUC0-t分别为（109.48±12.34）、（113.04±29.43）mg·h/kg，cmax分别为（38.76±1.62）、（42.28±2.03）mg/kg，组间差异无统计学意义（P＞0.05）。实验组小鼠ig左氧氟沙星的绝对生物利用度为（96.85±17.39）％。结论：iv痰热清注射液后iv或ig左氧氟沙星，左氧氟沙星在小鼠肺组织中的分布相似。%OBJECTIVE:To compare the distribution of levofloxacin in lung of mice after intragastric and intravenous adminis-tration of Tanreqing injection combined with levofloxacin. METHODS:144 mice were randomly divided into experimental group and control group,with 72 mice in each group. Both groups were given Tanreqing injection(0.8 ml/kg). 1 h later,control group was given Levofloxacin injection(80 mg/kg)intravenously,and experimental group Levofloxacin tablet solution(80 mg/kg)intra-gastrically;every 8 mice were sacrificed for lung tissue collection at the time points of 0.05,0.083,0.17,0.5,1.0,2.0,4.0,8.0, 12.0 h and 0.083,0.17,0.33,0.5,1.0,2.0,4.0,8.0,12.0 h,respectively. Using ciprofloxacin as internal standard,the content of levofloxacin in lung tissue was determined by HPLC,and pharmacokinetic parameters were calculated by 3p97 program. RE-SULTS:The concentration-time curves of levofloxacin in lung tissue were in line with two-compartment model in 2 groups. The main pharmacokinetics of experimental group and control group were as follows as t1/2β of(4.17±0.84)h and(4.10±0.55)h;CL of(0.66±0.049)L/h and(0.71±0.21)L/h;AUC0-t of(109.48±12.34)mg·h/kg and(113.04±29.43)mg·h/kg;cmax of(38.76± 1.62) mg/kg and (42.28 ± 2.03) mg/kg,respectively. There was no statistical significance (P>0.05). Absolute bioavailability of levofloxacin was(96.85±17.39)% in experimental group with intragastric administration. CONCLUSIONS:After intravenous injec-tion of Tanreqing injection,the distribution of levofloxacin in lung tissue of mice are similiar between intragastric administration and intragastric administration.