目的：探讨痰热清注射液对左氧氟沙星在小鼠肺组织中分布的影响。方法将144只小鼠分为痰热清注射液联合左氧氟沙星实验组和左氧氟沙星对照组，两组动物分别于0．05、0．083、0．17、0．5、1．0、2．0、4．0、8．0h和12．0h采集肺组织，制为匀浆，高效液相色谱法检测肺组织中左氧氟沙星药物浓度，3 P97程序计算药动学参数。结果实验组和对照组肺组织中药‐时曲线呈二室模型，实验组和对照组肺组织中V／Ft1／2β、CL ，AUCo‐t分别为（1．37±0．30 vs ．1．90±0．24）L、（5．21±1．96 vs ．4．82±1．55）h、（0．81±0．12 vs ．1．13±0．14）L／h和（117．69±22．89 vs ．84．91±11．33）mg · h－1· kg －1，组间差异有统计学意义（P＜0．05）。表明痰热清注射液能提高左氧氟沙星在小鼠肺组织中的分布量和滞留时间。结论痰热清注射液联合左氧氟沙星可提高左氧氟沙星在小鼠肺组织中的药物浓度并延长滞留时间长。%Objective To investigate the effects of Tanreging Injection on the distribution of levofloxacin in the lung tissue of mice .Methods 144 mice were randomily divided into the levofloxacin experimental group and the levofloxacin control group ,72 ca‐ses in each group .The experimental group was injected by Tanreqing Injection 0 .8 mg/kg and the control group was injected by the same volume of normal saline .The mice in the two groups were sacrificed for collecting the lung tissues at the time points of 0 .05 , 0 .08 ,0 .17 ,0 .5 ,1 .0 ,2 .0 ,4 .0 ,8 .0 ,12 h and preparing the homogenate .The levofloxacin concentration in the lung tissue was deter‐mined by HPLC and the pharmacokinetic parameters were calculated by the 3P97 program .Results The concentration curves based on drug‐time in the experimental group and the control group both fitted the two‐compartments model .The main pharmacokinetic parameters in the lung tissue V/Ft1/2β,CL ,AUCo‐t were (1 .37 ± 0 .30 vs .1 .90 ± 0 .24)L ,(5 .21 ± 1 .96 vs .4 .82 ± 1 .55) h ,(0 .81 ± 0 .12 vs .1 .13 ± 0 .14)L/h and (117 .69 ± 22 .89 vs .84 .91 ± 11 .33) mg · h-1 · kg -1 respectively ,the differences between the two groups were statistically significant (P<0 .05) .The results indicated that Tanreqing Injection increased the distribution amount of levofloxaction and residence time in the lung tissue of mice .Conclusion The combained of Tanreqing Injection and levofloxacin can increase the g concentration and residence time of levofloxacin in the lung tissue of mice .
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