RS4型抗性淀粉对高糖脂饮食大鼠糖脂代谢失衡的影响

摘要

以高脂饮食喂养的大鼠为实验动物模型,研究抗性淀粉干预高脂饮食诱发的代谢紊乱对大鼠血糖、血脂等相关代谢的影响.40只雄性Wistar大鼠随机分成正常组(Normal组)、高脂对照组(Control组)、普通酯化淀粉组(NCSA组)、蜡质酯化淀粉组(W]SA组)、高直链酯化淀粉组(RSA组),共5个实验组.6周造模结束后,与正常组相比,肥胖模型组大鼠体重、血糖、胰岛素(INS)、血脂水平在实验期间一直显著高于正常组,HDL-C水平显著低于正常组,且差异显著(P＜0.05);相比于对照组,灌胃普通酯化淀粉、蜡质酯化淀粉及高直链酯化淀粉能不同程度降低高脂饮食大鼠体重、血糖、胰岛素(INS)、血脂水平(HDL-C水平升高);蜡质酯化淀粉组对减缓大鼠体重增长、降低肥胖大鼠血糖、胰岛素(INS)和血脂水平效果最显著,与正常组无显著差异(P＞0.05).证明在高脂饲料喂养大鼠同时并灌胃蜡质酯化淀粉能有效的降低大鼠高脂饮食诱发的糖脂代谢紊乱.%In this experiment,rats fed with high-fat diet.The effect of resistant starch on the intervening of fat dietinduced metabolic disorders of blood glucose and lipid metabolism was studied.Forty male Wistar rats were randomly divided into five groups:normal group (Normal group),high fat control group (Control group),Common esterified starch group (NCSA group),waxy starch esterified group (WCSA group),and high amylose starch esterified group (RSA group).Various indexes were collected after 6 weeks.Compared with the normal group,the weight,blood glucose,insulin (INS),lipid levels of model group obese rats during the experiment was significantly higher than the normal group,HDL-C levels were significantly lower than the normal group with significantly (P ＜ 0.05) difference.Compared with the control group,NCSA group,WCSAgroup and RSA group all showedthe reduction of fat weight,blood glucose,insulin (INS),blood lipid diet of rats (HDL-C levels increasing) at varying degrees.The WCSA group showed the strongest activity to slow down the levels of weight gain of rats,lower blood sugar in obese,insulin (INS) and lipid.No significant difference was observed against the normal group (P ＞ 0.05).It was demonstrated that fedding high-fat diet and fed simultaneously esterified waxy starch could effectively reduce the glucose and lipid metabolism disorders of high-fat diet-induced rats.