酪蛋白酶解物中抗菌肽的抑菌性及稳定性研究

摘要

Studied on the stability of antibacterial peptides of the casein hydrolysate and their antibacterial performance. Using the Escherichia coli ,Staphylococcus aureus ,Bacillus subtelis and Bacillus cereus as indicator bacterias to study the antibacterial performance of antibacterial peptides of the casein hydrolysate .And used the Escherichia coli as indicator bacterias to study the stability of antibacterial peptides of the casein hydrolysate under the different temperature.different pH and different storage time .The result showed,antibacterial peptides of the casein hydrolysate can inhibit the Escherichia coli ,Staphylococcus aureus .Bacillus subtelis and Bacillus cereus with broad spectrum antibacterial properties.The antibacterial peptides of the casein hydrolysate had the stability toward high temperrature and also had a good stability of antibacterial performance toward Eschertchia coli under the alkaline condition.The antibacterial performance was enhanced under the acid condition.Their power form had good storability at the room terperrature.%对酪蛋白胰蛋白酶酶解物中抗菌肽的抗菌性及稳定性进行研究.实验通过以大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌和蜡样芽胞杆菌为指示菌,验证了抗菌肽的抑菌性,并以大肠杆菌为指示菌,研究了酶解物中抗菌肤在不同温度、不同pH值、不同储藏时间条件下的抑菌稳定性.结果表明:此抗菌肽能有效抑制大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌和蜡样芽胞杆菌的生长,具有广谱抗菌活性；碱性及中性条件下,此抗菌肽对大肠杆菌抑菌活性稳定,酸性条件下,抑菌活性随pH值下降而增强；此抗菌肽冻干样品在室温下可长期储藏.