以草鱼鱼糜漂洗液回收蛋白为原料,短肽得率和水解度为指标,从5 种常用蛋白酶中选出碱性蛋白酶作为酶解回收蛋白的最适酶,通过单因素和响应面优化试验得出最佳酶解条件:酶解温度为52.51 ℃,酶解pH 为9.06,酶添加量为1.42%,酶解时间为60 min,酶解底物蛋白浓度为1 g/dL.在此条件下回收蛋白的水解度为20.86%,短肽得率为73.5%;比较酶解前后回收蛋白的功能性质,发现酶解后多肽的溶解性、乳化性和起泡能力有所增加,乳化稳定性、泡沫稳定性和持油性较酶解前有所降低.%Proteins recovered from grass carp surimi wash liquid are as raw materials,alkaline protease is identified as the most suitable enzyme for enzymatic hydrolysis of protein among 5 commonly used enzymes based on simultaneous consideration of trichloroacetic acid nitrogen solubility index (TCA-NSI) and degree of hydrolysis (DH).According to single factor experiment and response surface methodology,the optimal enzyme reaction system is obtained as follows: hydrolysis temperature is 52.51 ℃,hydrolysis pH is 9.06,enzyme additive amount is 1.42%,hydrolysis time is 60 min and substrate concentration is 1 g/dL.Under the optimal hydrolysis conditions,the TCA-NSI and DH are 73.5% and 20.86%.The functional properties of protein before and after hydrolysis are compared;the results show that the solubility,emulsifying and foaming of protein have been increased after enzymatic hydrolysis than before.However,the emulsifying stability,oilabsorption capacity and foaming stability are decreased.
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