离子色谱法快速测定白酒中多种有机酸和阴离子

摘要

建立了梯度淋洗离子色谱法测定白酒中26种有机酸和阴离子的方法,研究了色谱柱、流速、pH值、淋洗液浓度等实验影响因素,确定了适于26种组分测定的多级梯度淋洗条件.结果表明,当流速为1.00 mL/min,pH值为5.2～6.5时,26种组分在0.02～2.00 mg/L质量浓度范围内具有良好的线性关系(R值均＞0.99).除了苯甲酸外,25种组分检出限(S/N=3)在0.000 11～0.072 80 mg/L;在0.50 mg/L、1.00 mg/L、2.00 mg/L三个添加浓度水平下,回收率为73.50％～115.13％,精密度实验结果相对标准偏差(RSD)为3.38％～4.97％.%A determination method of 26 organic acids and anions in Baijiu (Chinese liquor) was established by gradient elution ion chromatography.The experimental influence factors (chromatographic column,flow rate,pH and leacheate concentration) were researched,and then the multistage gradient elution conditions which were suitable for the determination of 26 components were determined.The results showed that in the conditions of flow rate 1.00 ml/min and pH 5.2-6.5,the linear relation of the 26 components was good in the range of 0.02-2.00 mg/L (R＞0.99).Except for benzoic acid,the limits of detection (S/N=3) of the 25 components were in the range of 0.000 11-0.072 80 mg/L.At the concentration addition levels of 0.50 mg/L,1.00 mg/L and 2.00 mg/L,the recovery rate was 73.50％-115.13％,the relative standard deviation (RSD) of precision tests was 3.38％-4.97％.