The optimum determination conditions of total polyphenols content in grape spirits by Folin-ciocalteaut method were researched.The results showed that the optimum determination conditions were be identified as reaction temperature 40 ℃,developing time 20 min and detection wavelength 736 nm.Under the optimum conditions,the gallic acid in the range of 50-400 μg/ml had a good linear relationship with the absorbance value (R2=0.997 4).The relative standard deviation (RSD) of method repeatability test and the precision tests results were 1.97% and 1.17%,respectively.The recovery rate was 98.61%-103.22%,RSD was 2.34%,which indicated the method had good repeatability,precision and high accuracy,and could be used for the determination of total polyphenols content in grape spirits.%该试验对福林-酚比色法测定葡萄烈酒中总多酚含量的最佳条件进行了研究.结果表明,最佳检测条件确定为反应温度40℃、显色时间20 min、检测波长736 nm.在此最佳条件下,没食子酸质量浓度在50~400μg/mL范围内与吸光度值线性关系良好(相关系数R2=0.9974),方法重复性试验和精密度试验结果相对标准偏差(RSD)分别为1.97%和1.17%,回收率为98.61%~103.22%,RSD值为2.34%,表明该方法重复性好、精密度和准确度较高,可用于葡萄烈酒中总多酚含量的检测.
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