干扰因子调控细菌纤维素微观结构影响研究

摘要

During the fermentation of Komagataeibacter sp.,the regulation and change of bacterial cellulose microstructure were investigated using dithiothreitol,chloramphenicol,Tween-80,emulsifier OP-10,quartz sand and nano-silica as interference factors.The results showed that interference factors could obviously change bacterial cellulose's network structure,but could not significantly affect the crystalline phase of bacterial cellulose.In the aspect of water holding capacity,Tween-80 could improve water content from 90 to 105 times of its own mass,dithiothreitol and OP-10 could decrease bacterial cellulose water content slightly,but chloramphenicol and nano-silica could decrease the water holding capacity to 70 times of its own mass.%在驹形杆菌发酵过程中,通过加入二硫苏糖醇、氯霉素、吐温-80、乳化剂OP-10、石英砂和纳米二氧化硅等干扰因子对其发酵过程进行结构调控,考察产物微观形态的变化.结果表明,干扰因子可以明显改变细菌纤维素的网络结构,但是却没有对细菌纤维素的晶型产生明显的影响.在持水性方面,吐温-80可以将细菌纤维素含水量从原有的90倍提高至105倍自身质量,二硫苏糖醇和OP-10会稍微降低细菌纤维素的含水量,氯霉素和纳米二氧化硅会将持水能力降至70倍自身质量.