中药与甲氧苄啶复方对小鼠血液学和免疫学指标的影响

摘要

为明确中药与甲氧苄啶复方在动物体内抗感染作用的机制,本试验研究了其对小鼠血液学和免疫学指标的影响.将受试药物按1、5、10 g/kg体重连续腹腔注射15d,通过CoulterJT血细胞分析仪对血液中的红细胞、白细胞、中性粒细胞、单核细胞和淋巴细胞进行了计数.采用脏器系数测定法、腹腔巨噬细胞吞噬鸡红细胞法、比浊法和MTT法分别对小鼠免疫器官指数、腹腔巨噬细胞吞噬功能、血清溶菌酶含量、血清溶血素水平和淋巴细胞增殖能力进行测定.结果表明中药与甲氧苄啶复方可显著增加血液中的红细胞数、单核细胞比例等(P＜0.05),显著增加免疫器官指数,增强腹腔巨噬细胞吞噬功能和血清溶菌酶活性,提高血清溶血素水平和淋巴细胞增殖能力(P＜0.05).因此中药与甲氧苄啶复方对小鼠非特异性免疫和特异性免疫功能均有增强作用.%To study the effect of Chinese herbs and Trimethoprim compound on immune function of mouse,and to further disclose the anti-infection mechanism in vivo of Chinese herbs and Trimethoprim compound. Drugs were tested injected into abdominal cavity according to 1,5,10 g/kg body weight for 15 d, the red blood cells, white blood cells,neutrophils,monocytes and lymphocytes were counted in blood of mice by Coulter-JT hematology analyzer. The index of immune organs, peritoneal macro-phages phagocytic function,serum lysozyme contents, serum hemolysin levels and lymphocyte proliferation were measured respectively, by using of organ coefficient method,macrophage phagocytic function test,turbidimetry and MTT method. The results showed that Chinese herbs and Trimethoprim compound could significantly increase the number of red blood cells, the proportion of monocytes in mice (P<0. 05). Chinese herbs and Trimethoprim compound could significantly increase the index of immune organs,enhance peritoneal macrophage phagocytosis and serum lysozyme activity,and improve the level of serum hemolysin and lymphocytes proliferative capacity(P<0. 05). The non-specific and specific immune function in mice were enhanced by Chinese herbs and Trimethoprim compound.