转基因生物检测技术研究进展

摘要

转基因技术的发展,促进了转基因生物(genetically modified organisms,GMO)的数量、多样性不断增加,给GMO检测工作提出了新的挑战,同时也促进了GMO检测方法的发展.GMO检测新方法突破了传统方法中一次只能分析一个靶目标的限制,新方法一次可以检测多个靶目标,向高通量检测的方向发展.作者对最新报道的GMO检测方法作一概述,主要包括实时荧光PCR多靶分析方法,锁式探针-基因芯片方法、液相芯片方法,多重PCR-毛细管电泳方法,生物发光检测方法,以及为GMO检测提供方法决策的支持系统(DSS、GMO示踪)等.%Progress in genetic engineering has led to the genetically modified organisms' (GMOs') increasing in amount and diversification, which pose a challenge in analysis of GMO and lead to the development of analytical methods for GMO detection. The new analytical technologies unlike the traditional approaches which mostly based on the sequential detection of one target at a time, use high-throughput systems or platforms for the detection of multiple targets once. The development of the newly developing tools such as real-time PCR-based ready-to-use multi-target analytical system, padlock probe ligation in combination with microarray detection PPLMD, Luminex xMAP technology, multiplex PCR-capillary gel electrophoresis, highly sensitive bioluminometric hybridization assay and decision support systems are briefly reviewed in this paper.