3种β2-受体激动剂荧光免疫层析试纸条的制备及特性研究

摘要

This study was aimed to prepare excellent fluorescence immunochromatographic strip for clenbuterol(CLB),ractopamine(RAC)and salbutamol(SAL),which could achieve the purpose of rapid and quantitative detection and laid a foundation for its industrialization.The properties of this strip including detection rage,detection limit,accuracy,degree of precision,false positive,false negative and coincidence with instrument method were investigated.The standard curve range of CLB,RAC and SAL test strips were 0 to 3.2,0 to 6.4 and 0 to 8.0 μg/L,respectively.The detection limit of CLB,RAC and SAL were up to 0.2,0.4 and 0.5 μg/L,respectively.The cross reaction between CLB,RAC,SAL and other eight similar drugs were negative which demonstrated the good specificity.The added recoveries of each batch were between 60％and 120％,which met the requirements.The coefficients of variation(CV)under different concentrations of CLB,RAC and SAL were less than 15％,which met the precision standard.Besides,the coincidence with instrument method was 100％.The strips was suitable for rapid detection of CLB,RAC and SAL residues in pig urine with the advantages of high sensitivity,good stability and strong specificity.%本研究旨在制备性能优异的克伦特罗(clenbuterol,CLB)、莱克多巴胺(ractopamine,RAC)和沙丁胺醇(salbutamol,SAL)荧光免疫层析快速定量检测试纸条,并对该试纸条的检测范围、检测限、准确度、精确度、假阳性、假阴性及其与仪器方法的符合率进行评价.结果显示,克伦特罗、莱克多巴胺和沙丁胺醇试纸条标准曲线范围分别为0～3.2、0～6.4、0～8.0 μg/L;克伦特罗、莱克多巴胺和沙丁胺醇试纸条检测下限分别可达0.2、0.4和0.5 μg/L;克伦特罗、莱克多巴胺和沙丁胺醇与其他8种同类药物交叉反应均成阴性,特异性良好;各批添加回收率在60％～120％之间,准确度达到要求;不同克伦特罗、莱克多巴胺和沙丁胺醇浓度下变异系数(CV)均小于15％,符合精密度标准;猪尿中假阳性率和假阴性率均为0;此外与仪器方法的符合率为100％.试纸条适用于猪尿中克伦特罗、沙丁胺醇和莱克多巴胺残留快速检测,具有灵敏度高、稳定性好和特异性强的特点.