白细胞介素-6快速定量荧光免疫层析试纸条的制备及特性研究

摘要

Objective To study a rapid and quantitative method to detect Interleukin-6(IL-6)by immuno-fluorescence technique.Methods The sensitivity and accuracy of strip associated with precision,specificity, stability properties were preliminarily evaluated.Results The standard curve showed a good linear(r2=0.965),LOD and LOQ of the strip were 8.49 pg/mL and 25.28 pg/mL.The average recoveries were 96.51%, 102.46% and 103.06%,respectively.The precision of intra-assay and inter-assay were less than 15.00%.And the cross-reaction with 7 competitors was less than 0.10%.Conclusion IL-6 immunochromatographic strip offered by our lab has better sensitivity,specificity and stability,as well as wide application prospect.%目的 利用免疫荧光技术研制一种快速、高灵敏度定量检测血清中白细胞介素-6(IL-6)的方法.方法 本研究对试剂盒的灵敏度、准确度、特异性和稳定性进行了初步的性能评价.结果 本方法的标准曲线线性关系良好,r2=0.965,检测线和定量限分别为8.49、25.28 pg/mL,添加回收试验测得其回收率平均值分别为96.51%、102.46%、103.06%,且制备试纸条的批间和批内精密度均小于15.00%,与7种竞争物交叉反应率小于0.10%.结论 本试验制备的IL-6定量荧光免疫层析试纸条的灵敏度高,特异性好,稳定性好,有较为广泛的应用前景.