MC顶空萃取-热解吸-GC/MS法研究香附指纹图谱

摘要

A novel solid-phase extraction element I. E. Magic-chemisorber( MC) combined with thermal desorption (TD)-gas chro* matography/mass spectrometry(GC/MS)was applied for the analysis of volatile component in Cyperus rotundas tubers from different growing areas. Conditions of the MC extraction and TD etc. Were optimized. The obtained fingerprints of 12 samples were based on the relative peak areas of 44 peaks in each GC profiles, and RSDs of the relative peak areas were less than 8.6%. The results showed that it was a simple, rapid, and effective method for the quality control of Cyperus rotundas tubers.%采用新型搅拌棒萃取器(MC)顶空萃取-热解吸-气相色谱/质谱(TD-GC/MS)方法分析香附中的挥发性成分,优化了MC萃取、解吸等条件,并对方法重现性进行考察,30个峰的相对峰面积的RSD均小于8.6％.继而测定了不同产地的香附样品,建立指纹谱图,标定44个共有峰,占总峰面积90％以上.并对图谱进行相似性评价及聚类分析,研究表明该法可为香附药材质量控制、产地选择提供理论依据.