Calvariutn cultured primary osteoblasts of new born SD rat were cultured and exposed to different concentrations of Astragalus total flavonoidsC ATF). The cell proliferation was detected by tetrazoline colorime-try(MTT) ,the differentiation was measured by activity of alkaline phosphatase(ALP) .levels of type I collagen proteins synthesis and osteocalcin production in the cell,the mineralization was evaluated by means of Alizarin Red-S staining,the contents of bone morphogenetic protein(BMP-2) was detected by ELISA method,and core binding factor α1(Cbfal, Runx-2) was detected by Western blot analysis. Results showed that proliferation,differentiation and mineralization of rat primary osteoblast were promoted by different concentrations of ATF in a dose-dependent manner,and ATF could increase the expression of BMP-2 and Runx-2. The results indicated that ATF could promote osteogenic activity of primary osteoblast by increasing the expression of BMP-2 and Runx-2.%在大鼠颅骨原代成骨细胞中加入不同浓度的黄芪总黄酮(ATF),用MTT法检测细胞增殖,用碱性磷酸酶(ALP)活性、Ⅰ型胶原及骨钙素水平检测细胞分化,用茜素红染色法检测细胞矿化,用ELISA法和Western blot方法检测成骨细胞中骨形成蛋白(BMP-2)及核心结合因子(Runx-2)的表达.结果表明,ATF能剂量依赖性地促进大鼠原代成骨细胞的增殖、分化及矿化,同时上调成骨细胞中BMP-2和Runx-2蛋白的表达.提示ATF可能通过上调成骨细胞中BMP-2和Runx-2的表达来促进成骨细胞的成骨活性.
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