LC-MS/MS 测定小鼠肝脏中甘草次酸含量

摘要

建立了 LC-MS/MS 测定小鼠肝脏中甘草次酸含量的方法。本法以咖啡酸为内标物质，采用多反应监测模式，对肝脏中甘草次酸进行含量测定。所建方法灵敏、准确、可靠，具有线性范围宽、专属性强、基质效应低、回收率及稳定性高的特点，可用于甘草次酸与雄黄联合用药小鼠肝脏中甘草次酸的含量测定。%A method of LC-MS/MS was established for the determination of glycyrrhetinic acid in liver of mice.Multiple reaction monitoring(MRM)scanning mode was used for the quantifi-cation with caffeic acid as the internal standard (IS).The method was validated for sensitivity, precision,reliability,a wide linear range,strong specificity,low matrix effect,high recovery and stability.The method was successfully applied for the determination of glycyrrhetinic acid in liver of mice combined treated with glycyrrhetinic acid and realgar.