LC-MS/MS DETERMINATION OF TACHYKININ-RELATED PEPTIDES IN THE CENTRAL NERVOUS SYSTEM REVEALS DIFFERENTIAL EXPRESSION IN TRPV1~(-/-) MICE

摘要

The LC-MS/MS analysis revealed that SP and NKA were significantly down-regulated in TRPV1~(-/-) spinal cord and brain tissues. These results clearly demonstrate that TRPV1~(-/-) mice exhibit lower SP and NKA concentrations in the central nervous system. Consequently, the observed deficits of thermal responses in TRPV1~(-/-) mice may also be related to the down-regulations of SP and NKA. In the spinal cord, these two peptides are associated to primary and secondary hyperalgesia.