以小麦麸皮为原料制备低聚木糖,先微波消解,后加酶水解,研究微波酶法制备低聚木糖过程中微波功率、微波处理时间、液料比、加酶量和酶解时间5个因素对低聚木糖提取率的影响.经单因素和正交试验,确定最佳工艺条件为:微波功率600 W、微波处理时间7 min、加酶量2.5％和酶解时间8h,低聚木糖提取率为47.5％.该工艺成本较低,所得到的粗提取液颜色较浅,便于脱色,明显优于传统单一的微波消解法和酶解法.%Using wheat bran as raw materials for preparation of xylo-oligosaccharide, first microwave digestion, and then enzymatic hydrolysis,the effect of 5 factors (microwave power, microwave irradiation time,liquid- solid ratio, enzyme concentration and hydrolysis time) on xylo-oligosaccharides extraction rate was studied. Through single factor and orthogonal test, the optimum process conditions were determined as that: microwave power of 600 W,treatment time of 7 min, enzyme concentration of 2. 5% and hydrolysis time of 8 h, the xylo-oligosaccharide extraction rate was 47. 5%. The process had low cost, the color of crude extract obtained was shallow, easy to decolour, which was obviously superior to the traditional microwave digestion method and enzyme hydrolysis method.
展开▼
