选用啤酒酵母为原料，采用热水浸提提取还原型谷胱甘肽（GSH），经过高速离心分离、阳离子交换树脂纯化、等电点沉淀及真空浓缩干燥制备还原型谷胱甘肽。采用1，1-二苯基-2-苦肼基（DPPH）自由基反应体系，抗氧化性物质抗坏血酸作为参照物，根据DPPH·体系吸光值的变化研究GSH清除自由基的能力，DPPH·反应体系为5mL 0.0817mmol/L DPPH·溶液+5mL样品溶液，反应时间30min。考察了不同浓度GSH和抗坏血酸与DPPH·自由基清除率的关系。结果表明，GSH比抗坏血酸抗氧化能力差一些，但GSH清除DPPH自由基能力与其浓度呈明显的量效关系，因此，啤酒酵母GSH有重要的抗氧化开发价值。%Reduced glutathione(GSH) was extracted from beer yeast with hot water and prepared through the processes including high-speed centrifugation, cation exchange resin purification, isoelectric precipitation and vacuum concentration and drying. Using the DPPH · radical reaction system and with ascorbic acid as the reference material, GSH’s ability of scavenging free radicals was studied according to the change of absorbance in the DPPH system. The DPPH reaction system was comprised of 5mL 0.0817mmol/L DPPH solution + 5mL sample solution, and the reaction time was 30min. The relationship between different concentrations of GSH and ascorbic acid with DPPH · free radical scavenging efficiency was investigated. The results showed that GSH was inferior to ascorbic acid in antioxidant ability, but an obvious dose-effect relationship existed between its ability of scavenging DPPH · free radicals and its concentration. So, the antioxidant ability of GSH in beer yeast is worth developing.
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