菌草种质资源SRAP多样性分析

摘要

该试验利用SRAP分子标记技术分析了48份菌草种质资源的遗传多样性,首先从256个组合中通过分析4个菌草材料择优筛选出18个扩增稳定、清晰、多态性较好的引物组合,然后用这18个组合分析了所有48份菌草种质资源材料,共获得了284个多态DNA片段,平均每个组合给出16个片段.UPGMA聚类分析结果表明:供试材料之间的遗传相似性系数(SM)分布在0.58～0.98之间,在遗传相似系数0.645时可以将48份菌草种质材料分成Ⅰ、Ⅱ、Ⅲ和Ⅳ四大类群,表明SRAP分子标记可准确、有效地用于菌草种质资源的遗传多样性分析,同时也表明我国各地还蕴藏着丰富的芦竹的种植资源有待于进进一步的采集和挖掘.该研究为菌草种质资源多样性分析提供了一种新的技术手段.未来可以利用该研究中筛选出的优良引物组合来分析更多、更广的菌草种质资源材料,为各类菌草种质资源植物的收集、分类、管理和开发利用提供更加全面的科学数据和理论依据.%SRAP (Sequence-related amplified polymorphism),a PCR-based molecular marker system was used to evaluate the genetic diversity of 48 JUCAO germplasms in this study.At first,256 SRAP primer pairs were used to amplify four representative germplasms to screen the best primer pairs that could produce polymorphic DNA fragments.As a result,a total of 284 polymorphic DNA fragments were scored among the 48 JUNCAO germplasms from the electrophoresis patterns generated with 18 selected SRAP primer pairs.By using NTSYS-pc 2.1 software combined with UPGMA cluster analysis,the genetic similarity (SM) coefficient between the 48 accessions was calculated and ranged from 0.58 to 0.98.All the 48 accessions were distinguished from each other.The 48 JUNCAO germplasms were classified into four categories at a genetic similarity coefficient of 0.645.The results showed that the SRAP markers could be effectively used for analysis of genetic diversity of JUNCAO germplasms.Additionally,the results also showed that there exist abundant JUNCAO germplasm in the genus Arundo in China.This study provides a new technique for studying the genetic diversity of JUNCAO germplasms.The selected combinations of SRAP primers can be used for genetic analyses on a larger number of JUNCAO germplasms in the future.Moreover,the results provide theoretical guidance and technical support for the efficient management and utilization of JUNCAO germplasm resources.