高效液相色谱法测定稻米和稻田水土中多菌灵残留

摘要

A method for determining carbendazim residue in water,soil,and rice was developed by HPLC using external standard method for quantitation.Samples were extracted with the mixed solution of methanol and dilute hydrochloric acid.The extracts cleaned up by liquid to liquid separation.The relation of carbendazim peak area and sample injective quality exhibited linearity in a range between 0.15 ng and 220 ng.The minimum detection mass ratio of carbendazim for paddy water was 0.01 mg/kg,for soils 0.01 mg/kg and for rice 0.02 mg/kg.Test results of spiked recoveries of 3 levels indicated that average spiked recoveries of carbendazim in paddy water ranged between 87.1% and 93.0% ,in soils between 84.8% and 91.9% ,and in rice between 83.9% and 89.6% .The RSD of carbendazim in paddy water ranged 3.3% and 3.8% ,in soils between 1.4% and 4.1%,and in rice between 1.8% and 5.5% .%采用高效液相色谱法测定稻米和稻田水土中的多菌灵残留,用稀盐酸溶液提取,经液液分配净化,外标法定量.多菌灵的峰面积与进样质量在0.15 ng～220 ng范围内呈线性相关,在稻田水、土壤和稻米中的最低检出质量比分别为0.01 mg/kg、0.01 mg/kg和0.02 mg/kg.3个质量水平的加标回收试验结果表明,多菌灵在稻田水样中的平均回收率为87.1％ ～93.0％,RSD为3.3％～3.8％;在土壤中的平均回收率为84.8％～91.9％,RSD为1.4％～4.1％;在稻米中的平均回收率为83.9％ ～89.6％,RSD为1.8％～5.5％.