亚硒酸钠对贫铀气管灌注大鼠免疫损伤的防护作用

摘要

Objective To investigate the hepatoprotective effect of total triterpenoids of Prunella vulgaris L. ( TTP ) on rats. Methods Sixty rats were randomly divided into six groups as follows: control group, CC14 group, TTP ( 62. 5 , 125 and 250 mg/kg ) groups and bifendate ( 200 mg/kg ) group. Rat acute liver injury model was induced by peritoneal injection of 50% CC14( 2 ml/kg, dissolved in corn oil ). Rats in therapeutic groups were pretreated with TTP or bifendate six days before CC14 was given. The effects of TTP on serum levels of alanine aminotransferase ( ALT ) and aspartate aminotransferase ( AST ), hepatic malondialdehyde ( MDA ) content, glutathione peroxidase ( GSH-Px ) and superoxide dismutase ( SOD ) activities in different groups were investigated. In addition, his-topathologic changes of the liver were detected with hematoxylin-eosin ( HE ). CYP2E1 protein and mRNA expressions in liver tissue were evaluated by Western blotting, immunohistochemical staining and transcriptase-polymerase chain reaction, respectively. Results TTP effectively decreased serum ALT, AST levels, hepatic MDA content, and restored hepatic SOD and GSH-Px activities in acute liver injury rats. Furthermore, TTP reduced CYP2E1 expression at both protein and mRNA levels in a dose-dependent manner. Conclusion TTP had protective and therapeutic effect on rat liver injury, which might be associated with its antioxidant properties and inhibition of CYP2E1 activation.%目的 观察亚硒酸钠对贫铀(DU)气管灌注大鼠免疫损伤的防护作用.方法 225只Wistar大鼠随机分为正常对照组、DU染毒组、DU+低硒组、DU+中硒组、DU+高硒组,每组45只.采用单次气管灌注贫铀悬液500 μg/只染毒,DU+低硒组、DU+中硒组、DU+高硒组自DU染毒前3天灌喂分别给予50、200、500 μg/kg的硒溶液,每天一次直至活杀.分别在气管灌注后第7、14、28天活杀大鼠,分别测定体重、肺铀含量、外周血细胞参数、T淋巴细胞及其亚群、血清免疫球蛋白IgG.结果 DU染毒组大鼠早期体重下降、白细胞数升高、红细胞数、T淋巴细胞总数及免疫球蛋白IgG显著降低;DU+低硒组、DU+中硒组、DU+高硒组各项指标与DU染毒组比较有明显改善.结论 亚硒酸钠对DU气管灌注大鼠免疫损伤有防护作用.