辽五味子果实挥发油成分的鉴定

摘要

目的对辽五味子的挥发油成分进行鉴定。方法利用色质联用仪GC/MS产生的双线性数据，藉化学计量学发展的二维色谱/质谱数据解析方法，得到了各组分的纯色谱曲线和质谱；根据色谱保留时间，同时利用解析所得的纯物质质谱在NIST质谱库进行相似检索对各个组分进行定性分析，最后利用总体积积分法得到定量结果。结果在辽五味子干燥果实挥发油中共检出56个组分，鉴定了其中49个化合物，占挥发油总量的98.27%。结论此法大大提高了定性定量分析的准确度，成功地检测出一些色谱分离不完全和低含量的组分。%AIM　To detect chemical components of the essential oil of Schisandra chinensis(Turcz.)Baill.. METHODS　The essential oil was analyzed with GC/MS, heuristic evolving latent projections(HELP) resolution and overall volume integration method. RESULTS　HELP method along with the data from GC/MS can be used to conduct the peak purity examination and resolution of overlapping peaks to obtain pure chromatogram and MS spectrum of each component. Fifty six components were separated and 49 of them were qualitatively and quantitatively analyzed. All the components represent about 98.27% of the total content. CONCLUSION　The resolved pure chromatogram and MS spectrum can greatly enhance the reliability of similar searches in the commercial MS database and thus contribute to the accuracy of the qualitative and quantitative analysis of the essential oil.