甲鱼蛋白抗氧化肽的中性蛋白酶酶解条件优化

摘要

采用甲鱼蛋白为原料制备抗氧化活性肽，以DPPH（1，1-二苯基-2-三硝基苯肼）清除率和DH（水解度）为评价指标，在单因素试验的基础上，采用响应面法优化了中性蛋白酶酶解甲鱼蛋白制备抗氧化肽的酶解条件。结果表明：温度50.65℃，酶解 pH 值7.48，加酶量2.9％时， DPPH 清除率最高，为（85.56±1.52）％，对应的水解度为（7.68±0.52）％。%Antioxidant activity peptides was prepared with turtle as raw material .In order to optimize the enzymatic hydrolysis condition of turtle protein, based on the single-factor experiment, a mathematics model has been established by Response Surface Methodology (RSM), and the validity of the model has been validated .Results showed the better antioxidant activity peptide were obtained when the hydrolysis temperature was 50.65℃, pH at 7.48, catalyzer addition was 2.9%.In above optimized condition, the DPPH cleanup rate and DH were reached to 85.56%±1.52%and 7.68%±0.52%, respectively.