冷鲜鸡污染微生物磺胺类和喹诺酮类耐药基因分析

摘要

动物养殖业的发展过程中抗生素的长期使用,导致耐药菌的快速发展.这些耐药菌在动物养殖过程中逐渐被抗生素筛选,在动物性食品生产链中容易被携带传播,对人类食品安全产生很大的威胁.试验选取浙江省绍兴市8个大型超市和11个农贸市场销售的冷鲜鸡为检测对象,采用PCR和测序验证的技术手段,对冷鲜鸡表面微生物中的2种磺胺类耐药基因sulⅠ、sulⅡ和8种喹诺酮类基因qnrA、qnrB、qnrC、qnrD、qnrS、qepA、oqxA与oqxB进行检测.在所检测的样品中,检测出sulⅠ、sulⅡ、qnrD、qnrS、qepA和oqxB基因,检出率分别为100%、100%、100%、89.5%、100%和89.5%.并对所检出的耐药基因进行测序和NCBI收录的耐药基因序列进行Blast比对验证,比对结果同源性分别为100%、99.44%、100%、92.74%、99.82%和100%.试验结果将为冷鲜鸡中耐药基因的风险评估提供有力支持.%The long-term use of antibiotics in the animal farming industry has caused rapid expansion of drug resistant bacteria.These drug resistant bacteria are screened during the process of animal farming,and are easily transmitted in the animal production chain,which is a huge threat to human's food safety.In this study,we aimed at testing the safety of refrigerated chicken collected from eight supermarkets and eleven agricultural markets in Shaoxing city,Zhejiang Province.We detected the antibiotic genes of sulfonamides and quinolones of surface microorganisms of the samples by PCR reaction and sequencing.We detected sul Ⅰ,sulⅡ,qnrD,qnrS,qepA and oqxB in the samples and the detection rate was 100%,100%,100%,89.5%,100% and 89.5%,respectively.We also sequenced the antibiotic genes with the sequences in NCBI and the coincidence rate was 100%,99.44%,100%,92.74%,99.82% and 100%,respectively.This study will provide effective support for risk assessment of antibiotic genes in the refrigerated chicken.