蛹虫草凝集素编码基因JRL-ccm4的初步研究

摘要

通过对蛹虫草基因组数据挖掘，发现Jacalin类凝集素编码基因JRL-ccm4，进而对该基因及其编码产物进行了生物信息学分析，通过原核异源表达获得凝集素蛋白JRL-ccm4，并检测了表达产物的生物学活性测定；通过RT-PCR对不同生长时期的蛹虫草样品中 JRL-ccm4基因的表达水平进行了检测。研究结果表明：JRL-ccm4蛋白有一个JRL结构域，能在原核细胞中成功表达；异源表达的JRL-ccm4蛋白具有抑制金黄色葡萄球菌的活性，有一定抑制人宫颈癌细胞 HeLa 增殖的活性；原基期 JRL-ccm4基因表达水平最高，是菌丝期的3.49倍，是子实体时期的2.39倍，表明JRL-ccm4基因可能参与蛹虫草子实体形成过程。%Through the Cordyceps militaris genome data mining,a lectin gene encoding JRL-ccm4 was found.In this research,the gene and its encoding product were analyzed by bioinformatics,and heterologously expressed and examined the biological activity of the protein in Escherichia coli.The JRL-ccm4 gene expression level in different growth periods of C.militaris was tested by qRT-PCR.The results showed that the JRL-ccm4 proteins had a JRL domain structure which could be successfully expressed in prokaryotic cells.The protein of the JRL-ccm4 could inhibit growth of Staphylococcus aureus and partly inhibit cervical cancer HeLa cell proliferation;the JRL-ccm4 had the highest amount of gene expression in stromata,which was 3 .49 times that of hyphae period and 2.39 times in the period of the fruiting body.It suggested that the gene could be related to fruit body development.