商洛绿茶茶多酚制备工艺优化及其抗氧化活性研究

摘要

采用正交试验法对商洛绿茶茶多酚的制备工艺进行了优化，并从DPPH自由基、羟基自由基、超氧阴离子、ABTS自由基和总还原力方面分别检测了商洛绿茶茶多酚的体外抗氧化活性。研究表明：商洛绿茶茶多酚的最佳提取工艺条件为液料比20∶1、浸提温度60℃、乙醇浓度50％、浸提时间30 min；商洛绿茶茶多酚具有较强的抗氧化活性，并且呈现出良好的量－效关系。%The preparation technology of polyphenol from Shangluo green tea was optimized through orthogonal test, and its an-tioxidant activity in vitro was determined from the aspects of DPPH free radical, hydroxyl radical, superoxide anion, ABTS free radi-cal and total reducing power.The optimum extraction process conditions for Shangluo green tea polyphenol were acquired as follows:liquid-material ratio 20∶1, extraction temperature 60 ℃, ethanol concentration 50%, and extraction time 30 min.The Shangluo green tea polyphenol had a strong antioxidant activity, which showed a good dose-response relationship.