猕猴桃果肉提取物抗氧化活性研究

摘要

By measuring systematically in simulated chemical systems through the ways of scavenging DPPH , ABTS and superoxide anion radicals and FRAP method ,the antioxidant activity of acetone-extracting from kiwifruits pulp with flash extraction and the content of the total phenol and total flavonoids of the extract were measured .The results showed that the content of total phenol and total flavonoids of the extract were (3.19 ±0.53) mg/g and (0.26 ±0.07)mg/g,respectively.The research in simulated chemical systems showed that the acetone-extracting from kiwifruits had great scavenging effect against・ OH,DPPH・ and O 2-・ in the experimental range.When the con-centrate of sample was 1 g/mL,the scavenging rate to superoxide anion radicals was up to 90.7%.And when the concentrate was 2 g/mL,the scavenging rate to ・ OH and DPPH・ was 84.5%and 82.2%,respectively.With the increasing concentration,the scavenging effect enhanced,which suggested the presence of dose-effect relations.Be-side,the FRAP value of acetone-extracting from kiwifruits also increased with concentration ,which had excellent significant correlation with its DPPH・ and O 2-・ radical-scavenging activity,significant correlation with its・ OH rad-ical-scavenging activity.The results provide the theory basic for kiwifruits exploitation .%　　以猕猴桃果肉为原料，利用闪式提取法制备猕猴桃丙酮提取物，在化学模拟体系下，采用清除・ OH法、DP-PH・法、O 2-・法以及FRAP法4种方法对猕猴桃丙酮提取物的抗氧化活性进行分析，并测定其中总酚以及总黄酮的含量。结果表明，猕猴桃丙酮提取物中总酚含量为（3．19±0．53）mg/g，总黄酮含量为（0．26±0．07）mg/g，在试验所选取的浓度范围内，具有良好的清除・ OH、DPPH・、O 2-・的作用，以及较强的还原能力，且呈现剂量效应关系。2 g/mL猕猴桃丙酮提取物对・ OH和DPPH・的清除率分别达到84．5％和82．2％，当浓度为1 g/mL时，对O 2-・的清除率高达90．7％。猕猴桃丙酮提取物的还原力与其清除DPPH・和O 2-・的能力呈现出极显著的相关性，与其清除超・ OH的能力呈现出显著的相关性。试验结果为综合开发利用猕猴桃资源提供了理论基础与科学依据。