声明
LIST OF ABBREVIATION
LIST OFFIGURES
LIST OFTABLE
CHAPTER 1 INTRODUCTION
1.1 Background and Motivation
1.2 Literature Review on Medicinal Plant
1.3 Overview of Codonopsis Pilosula (Dangshen)
1.3.1Radix Codonopsis Pilosula
1.3.2Distribution of C.Pilosula
1.3.3Pharmacological Activity of C. Pilosula
1.3.4Morphological Characteristic of Codonopsis Pilosula
1.4 Literature Review on Polysaccharides
1.4.1 Category of Polysaccharides
1.4.2Extraction Methods of Polysaccharides
1.4.3Monosaccharide Composition Analysis of Polysaccharides
1.4.4Physicochemical Properties of Polysaccharides
1.4.5Molecular Weight Determination of Polysaccharides
1.5 Characterization of Crude Polysaccharides
1.5.1Dynamic Light Scattering
1.5.2Circular Dichroism Spectroscopy
1.5.3The Congo Red Examination Test
1.5.4X-Ray Diffraction System
1.5.5Observation by Scanning Electron Microscope
1.6 Antioxidant Activity Assays of Polysaccharides
1.7 Project Aims
CHAPTER2EXTRACTION, OPTIMIZATION,AND PHYSIOCHEMICAL PROPERTIES DETERMINATION OF C. PILOSULA POLYSACCHARIDES
2.1 Test Materials
2.1.1 Sample Collection
2.1.2 Test Reagent
2.1.3 Test Instruments
2.2 Test Methods
2.2.1 Sample Preparation
2.2.2Hot-Water Extraction Methods of C. Pilosula Polysaccharides
2.2.3Optimization Extraction Condition of CPPs
2.2.4 Physicochemical Properties Analysis
2.2.5Determination of Molecular Weights of HW-CPPs
2.2.6 Monosaccharide Analysis
2.2.7 Statistical Analysis
2.3 Results Analysis
2.3.1SingleFactors Experiments
2.3.2Optimization of extraction conditions of HW-CPPs
2.3.3Physicochemical Properties Analysis of HW-CPPs
2.3.4Molecular Weight Analysis of HW-CPPs
2.3.5Monosaccharide Composition Analysis
2.4 Discussion
2.5 Summary
CHAPTER 3 CHARACTERIZATION OF C. PILOSULA POLYSACCHARIDES
3.1Test Materials
3.1.1 Sample Preparation
3.1.2Test Reagents
3.1.3 Test Instruments
3.2Test Methods
3.2.1Spectroscopic Analysis of HW-CPPs
3.2.2Dynamic Light Scattering Analysis of HW-CPPs
3.2.3Conformational Structure Analysis of HW-CPPs
3.2.4Circular Dichroism Spectroscopy Analysis of HW-CPPs
3.2.5Scanning Electron Microscope Observation of HW-CPPs
3.2.6X-Ray Diffraction Analysis of HW-CPPs
3.3 Results
3.3.1Fluorescence Spectra Analysis of HW-CPPs
3.3.2 Dynamic Light Scattering Analysis of HW-CPPs
3.3.3 Conformational Structure Analysis
3.3.4CircularDichroism Spectroscopy Analysis of HW-CPPs
3.3.5Scanning Electron Microscope Analysis of HW-CPPs
3.3.6X-ray Diffraction Analysis of HW-CPPs
3.4 Discussion
3.5 Summary
CHAPTER 4 DETERMINATION OF ANTIOXIDANT ACTIVITY OF C. PILOSULA POLYSACCHARIDES
4.1Test Materials
4.1.1 Sample Preparation
4.1.2 Test Reagents
4.2Test Methods
4.2.1Determination of the Antioxidant Activity of HW-CPPs
4.3Results analysis
4.3.1Determination of Antioxidant Activity
4.5 Discussion
4.6Conclusion and Future Work
参考文献
DEDICATION
致谢
LIST OF PUBLICATIONS
兰州理工大学;
