Recombinant fibrinogen synthesis: transient transfection CHO cells in suspension

摘要

Fibrinogen Aα chain can be transfected to CHO_(β γ) cells to produce significant quantity of secreted, detectable fibrinogen. Cell concentration can attain 6 million cells per ml 10 days after transfection and cell viability is still higher than 50%. It provides a means for the future production of recombinant chimeric fibrinogen and direct incorporation, at the genetic level, of protein therapeutics into fibrin matrices. The optimal production and growth medium for CHO_(β γ) cells still requires additional development and these data will be presented.