Study on Kinetics of Protein Forward Extraction from Quinoa by AOT/Isooctane Reverse Micelles System

摘要

In this paper, the kinetic process of extracting total protein from quinoa by AOT/isooctane reverse micelles was systematically studied. The effects of extraction time, extraction temperature, particle size and oscillation rate on quinoa protein forward extraction rate were Investigated respectively. Results showed that extraction of quinoa protein by AOT/isooctane reverse micelles system was mainly controlled by Internal diffusion, that is, the diffusion of protein from the interior of the particle to the surface was the control step of the reverse micelle extraction process. The extraction process can be explained by shrinking core model. The macroscopic dynamic equation of the protein forward extraction by the reverse micelles system was established and verified by extraction experiments at different temperatures. The experimental results indicated that the extraction process belonged to first order reaction and the apparent activation energy was 14.65kJ/mol. The kinetic equation model could better describe the reverse micelle extraction process of quinoa protein and could provide important theoretical reference for the comprehensive development and utilization of quinoa protein in the future.