The extraction of peanut protein peptone hydrolysate from cold pressed peanut meal by Papain

摘要

This paper studied the extraction of peanut protein peptone hydrolysate from cold pressed peanut meal by papain to provide a theoretical basis on peanut meal special microorganism culture makings products for further research. The study use orthogonal test method to optimize the method of papain preparation of peanut peptone, the best preparation conditions were: enzymolysis temperature 55°C, pH7.5, substrate concentration 3%, the amount of enzyme 6%, enzymolysis time 6 hours. The Protein yield was 52.02% and the content of amino nitrogen was 0.772 mg% by this perparation condition. Peanuts are one of our country and the worldwide economic crops; they have higher nutrition value and economic value. Fat and protein are the first and second largest in the peanut nutrient. At present, the peanut processing mainly oil, and to the use of peanut protein is quite limited. Now people more and more focuse on the use of peanut protein. The traditional peanut hot press process in our country can make serious degeneration of peanut protein, peanut meal most is used for feed use. Peanut oil cold pressing technology in recent years successfully solved the peanut protein serious degeneration of this technical problem in the process of oil, and can make the proteins and fats get efficient separation. Therefore the peanut protein as a byproduct of after peanut oil by make full use of and gradually come into the mind of the people's attention. Cold pressed peanut meal in hulling catsup as raw material, extracted by cold pressing technology after oil by-product. Peanut meal for white, pale peanut fragrance, shape of powder, at present is mainly used for feed. Peptone is a protein hydrolysis by enzyme, acid and alkali, a mixture of peptide and amino acid composition. It as the basis of microbial culture medium the main components, is microbial survival, growth and reproduction of the main source of nitrogen nutrition. In the pharmaceutical industry, fermentation industry, biological products and dosage in the field of microbiology, etc all big; In the international market, peptone belongs to the cargo price, one of the varieties of short-term. Using enzyme technology to digestion using low value of peanut protein resource is an important way of promoting the development of peanut protein resources. Cold pressed peanut meal protein content as high as 56% - 60%, producing peptone is as good as raw materials. So the development of cold pressed peanut peptone and extends the peanut industry chain, promoting resources added value is to realize the peanut resources sustainable development key technical problems to be solved.