SYNTHESIS OF THE PELLICLE REPEATING UNITS OF LACTOCOCCUS LACTIS STRAINS FOR INVESTIGATION OF LACTOCOCCAL PHAGE INFECTION

摘要

Lactococcus lactis, a gram-positive bacteria, is widely used by the dairy industry due to its innate ability to produce lactic acid via lactose fermentation. The bacteria is encased in a strain specific polysaccharide pellicle, typically composed of hexasaccharide repeating units bridged by phosphodiester bonds [1]. These pellicles function as protective barriers against host phagocytosis, however lactococcal bacteriophages have been found to bind to these carbohydrate moieties via Receptor Binding Proteins (RBPs). Infection can lead to delayed fermentation, alteration of product quality and, in severe cases, loss of product; all resulting in considerable economic loss [2]. Understanding the recognition and binding processes involved in lactococcal bacteriophage infection is key to the prevention and control of its occurrence.