SYNTHESIS OF dUTPase DERIVED PEPTIDES FOR GENERATION OF ENZYME SPECIFIC ANTIBODIES

摘要

The recently identified uracil-DNA specific nuclease is the first representative of a new family of nucleases (Vertessy et al, submitted). This enzyme contributes to survival of multiresistant cancer cells, while its inhibition induces thymine-less cell death in this way a promising anti-cancer strategy. To analyze the physiological function of this protein, peptide conjugates were prepared to serve as synthetic antigens for the generation of antibodies against various isoforms of dUTPase.