Antibody Specificity to Oncogene-Derived Peptides for Breast Cancer Detection

摘要

This report describes the work carried out on the specificity of four oncogenicpeptide-monoclonal antibody interactions. Monoclonal antibody 172-12A4, which was raised against a peptide from v-erbB was assayed by ELISA with a set of 152 substitution analogs of the antigenic determinant to yield a fingerprint profile of this antigen-antibody interaction. A number of peptide libraries have also been screened and the deconvolution of individual sequences is ongoing. Peptide libraries have been screened against mAb 121-15B10, which was raised against a peptide from v-fes and is homologous with the v-erbB peptide. Peptides with nanomolar affinities have been identified. The antigenic determinants for six antibodies against two different oncogenic peptides were located using omission analogs. Substitution analogs are being synthesized to identify the amino acid specificities of each of these antibodies. Peptide libraries were also screened against these two peptide-antibody interactions, and the deconvolution process is ongoing. The understanding of antibody specificity at the amino acid level will help in the development of these antibodies as early diagnostics for breast cancer.