首页> 外文会议>International Conference on Solid-State Sensors, Actuators and Microsystems >ENHANCED RADIAL DISPERSION AS A MEANS TO GAIN SEPARATION PERFORMANCE IN MINIATURIZED LIQUID CHROMATOGRAPHY SYSTEMS
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ENHANCED RADIAL DISPERSION AS A MEANS TO GAIN SEPARATION PERFORMANCE IN MINIATURIZED LIQUID CHROMATOGRAPHY SYSTEMS

机译:增强径向分散体作为在小型化液相色谱系统中获得分离性能的手段

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In the current contribution, it is demonstrated that radial elongation of support structures in a chromatographic column has an enormous impact on the axial band broadening occurring in the column. By increasing the lateral dimension of the support structures with respect to their axial dimension, axial band broadening could be reduced up to 5 times. For flat rectangular columns filled with 5 μm axial length support structures and a spacing of 2.5 μm, the minimal (apparent) plate height could be reduced from 2.6 to 0.5 μm under non-retained conditions by increasing the ratio of the lateral over the axial dimension from 1.2 to 15. This increase in performance can be addressed to a decrease in longitudinal dispersion, which was reflected in a reduced B-term by a factor of 25. The footprint of the column is dramatically reduced, ending up with an equivalence of 2 million plates per meter for an aspect ratio of 15. Under retained conditions, a 4 component (Coumarin) separation could be demonstrated requiring 1 mm channel length only.
机译:在目前的贡献中,证明了色谱柱中的支撑结构的径向伸长率对柱中发生的轴向带宽产生巨大影响。通过增加支持结构相对于其轴向尺寸的横向尺寸,轴向带宽化可以减少5倍。对于填充有5μm的轴向长度的支撑结构和2.5μm的间距扁平的矩形柱,最小(表观的)板的高度可以从2.6非保留的条件下,通过增加横向的比值超过轴向尺寸减小到0.5微米从1.2〜15.这种性能的增加可以解决纵向分散的减少,其在减少的B期反映为25倍。柱子的占地面积显着减少,最终达到2每米的百万平板纵横比为15.在保留条件下,可以证明仅需要1mm通道长度的4个组分(香豆素)分离。

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