Separation of Metal Ions (Fe(+3), CU(+2), Ni(+2), Zn(+2)) by Use of Bonded Phases in Thin-Layer Chromatography and High-Performance Liquid Chromatography

摘要

The purpose of this research was to obtain quick, chromatographic separations of Fe(+3), Cu(+2), Ni(+2) and Zn(+2) metal ions using thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) on bonded stationary phases and to compare the TLC separations with TLC separations obtained on plain silica gel. Eight bonded-phase substrates were synthesized, immobilizing amino, dithiocarbamate and beta-diketone groups on a silica surface. Separations were obtained on these bonded phases and on two types of silica using up to thirty mobile phases. An immobilized trifluoro beta-diketone substrate used with a 10% trifluoroacetylacetone in acetone mobile phase gave a well resolved separation of the four metals in TLC. An HPLC column packed with this substrate, and using the same mobile phase as in TLC, showed well resolved separation of Cu(+2), Zn(+2) and Ni(+2), Fe(+3) was not injected onto the column because of its extremely long retention time. Detection of the metals was accomplished by spraying with diphenylcarbazide for TLC and by visible and atomic absorption spectrometry for HPLC. (Author)