The development of the system for quantitative assessment of soya bean content in meat products by real time PCR

摘要

The object of this research was to develop the system for quantitative assessment of soya bean content in meat products (regardless of the type of their technological processing) by real time PCR. The search of the DNA template was conducted on the Internet using the open access genetic database of the US National Center for Biotechnological Information. CLC Sequence Viewer and Primer Express 2 were used for primer design. Specificity of the selected primers was studied theoretically using on-line the interactive system BLAST. DNA was extracted by the method based on DNA extraction and purification with ion detergent cetyltrimetylammonium bromide (CTAB) followed by sorption on a carrier. PCR was carried out on the ABI PRISM 7000 device with the detection of the amplification products in real time. In total, 8 sausage samples with a-priori known soya bean content were analysed. The quantitative PCR assay showed that the soya bean content in these samples corresponded to the amount of soya bean incorporated into the product according to the formulation ± 27% in dry or gel form. The obtained results confirm the possibility to use the system of soya bean quantitative assessment by real-time PCR to detect the possible falsification of the product or to confirm product authenticity in accordance with the regulations or the information on the label and in the accompanying documents.