首页> 外国专利> MEDIUM FOR CULTURING AND EXPANDING NEPHRON PROGENITOR CELLS, METHOD FOR CULTURING AND EXPANDING NEPHRON PROGENITOR CELLS, AND METHOD FOR PRODUCING RENAL ORGANOIDS

MEDIUM FOR CULTURING AND EXPANDING NEPHRON PROGENITOR CELLS, METHOD FOR CULTURING AND EXPANDING NEPHRON PROGENITOR CELLS, AND METHOD FOR PRODUCING RENAL ORGANOIDS

机译:培养和拓展肾祖细胞的培养基,培养和拓展肾祖细胞的方法,以及生产肾细胞体的方法

摘要

This medium is for culturing and expanding nephron progenitor cells and contains FGF2, heparin, a ROCK inhibitor, a GSK3β inhibitor, leukemia inhibitory factor (LIF), an ALK inhibitor, a BMP inhibitor, and a JAK inhibitor. This method is for culturing and expanding nephron progenitor cells by using said medium. This method for producing renal organoids comprises: a step for culturing and expanding a nephron progenitor culture by said culturing and expanding method using the medium; and a step for differentiating, into renal organoids, the nephron progenitor cells obtained through the culturing and expanding.
机译:该培养基用于培养和扩展肾祖细胞,含有FGF2,肝素,岩石抑制剂,GSK3β抑制剂,白血病抑制因子(LIF),ALK抑制剂,BMP抑制剂和JAK抑制剂。该方法用于通过使用所述介质培养和扩展肾血管祖细胞。这种制备肾细胞体的方法包括:使用培养基通过所述培养和膨胀方法培养和扩展肾祖培养的步骤;和通过培养和膨胀获得的肾子组织物细胞分化为肾细胞细胞的步骤。

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